Study type: Medical/biological study (experimental study)

Synthesis of diazoluminomelanin (DALM) in HL-60 cells for possible use as a cellular-level microwave dosimeter tech./dosim.

By: Bruno JG, Kiel JL
Published in: Bioelectromagnetics 1994; 15 (4): 315-328
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Aim of study (acc. to author)

To examine the thermalization and light production (i.e. chemiluminescense) of mammalian cell lysates in vitro during 2450 MHz microwave irradiation. Cells were treated before with a special chemical compound (DALM) (abbreviation: DALM = diazoluminomelanin, an radiofrequency-sensitive luminescent polymer).

Background/further details

Previous data (Kiel 1983) suggested that luminescent organic materials might act as microwave dosimeters within cells.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: 10 min
  • power: 20 W maximum (0, 3, 20 W)

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Polarization
  • circular
Exposure duration 10 min
Modulation
Modulation type CW
Exposure setup
Exposure source
  • not specified
  • model 15222 RF generator (MCL Inc., Bolingbrook, IL)
Chamber polysterene cuvettes 1x1x4,5cm, fixed onto fiber optic pipe
Parameters
Measurand Value Type Method Mass Remarks
power 20 W maximum cf. remarks - 0, 3, 20 W

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • during exposure

Main outcome of study (acc. to author)

The lysate of HL-60 cell line containing DALM showed an increased chemiluminescense with inreased temperature and microwave power input, respectively.
The data illustrate the potential of using DALM synthesized in mammalian cells as a photon-emitting sensor of absorbed energy in radiofrequency irradiation dosimetry.

Study character:

Study funded by

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