EMF-Portal | Effect of 50 Hz electromagnetic fields on acid phosphatase activity

Effect of 50 Hz electromagnetic fields on acid phosphatase activity med./bio.

By: Prashanth KS, Chouhan TRS, Nadiger S

Published in: Afr J Biochem Res 2009; 3 (3): 60-65

Aim of study (acc. to author)

Background/further details

The enzyme activity was examined under different conditions: 1.) no treatments, 2.) magnetic field exposure, 3.) variations of pH value, 4.) variations of pH value + magnetic field exposure, 5.) variations of temperature and 6.) variations of temperature + magnetic field exposure.

Endpoint

Exposure

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 60 min	magnetic flux density: 0.53 mT effective value

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Exposure duration	continuous for 60 min

Exposure setup

Exposure source	Helmholtz coils
Setup	pair of Helmholtz coils mounted 7 cm apart on a wooden frame; each coil with an inner radius of 7 cm, an outer radius of 10 cm and 500 turns of copper wire with a diameter of 0.25 mm; at the center of the coils system a PVC test tube stand was placed to hold the samples

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.53 mT	effective value	measured	-	-

Exposed system:

isolated bio./chem. substance
acid phosphatase

Methods Endpoint/measurement parameters/methodology

Investigated system:

isolated bio./chem. substance

Time of investigation:

before exposure
after exposure

Main outcome of study (acc. to author)

Magnetic field exposure alone (group 2) led to a significantly increased Michaelis-Menten constant and a significantly increased maximum reaction velocity compared to the samples without treatment (group 1). No significant differences were observed for the optimal pH value (group 4 compared to group 3) and for the optimal temperature (group 6 compared to group 5) between exposed and unexposed samples.
The authors conclude that exposure to extremely low frequency magnetic fields increased the enzyme activity of the acid phosphatase.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

not stated/no funding

Afrasiabi A et al. (2014): Synaptosomal acetylcholinesterase activity variation pattern in the presence of electromagnetic fields

Zhang J et al. (2012): Effect of alternating magnetic field treatments on enzymatic parameters of cellulase

Batcioglu K et al. (2011): Investigation of a weak magnetic field effect on the in vitro catalytic activity of adenosine deaminase and xanthine oxidase

Prashanth KS et al. (2008): Effect of 50 Hz electromagnetic fields on Alpha amylase activity

Portaccio M et al. (2005): Modulation of the catalytic activity of free and immobilized peroxidase by extremely low frequency electromagnetic fields: dependence on frequency

Portaccio M et al. (2003): In vitro studies of the influence of ELF electromagnetic fields on the activity of soluble and insoluble peroxidase