Study type: Medical/biological study (experimental study)

Effects of pulsed electric fields on rat liver homogenate paramagnetic species med./bio.

By: Cannistraro S, Martino G, Sportelli L
Published in: Radiat Environ Biophys 1980; 18 (2): 123-128
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Aim of study (acc. to author)

To investigate the effects of pulsed electric fields on paramagnetic species of rat liver homogenate in vitro by using electron spin resonance spectroscopy.

Background/further details

The livers of 18 days old Wistar rats (320-340 g) were used.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 200 kHz
Modulation type: pulsed
Exposure duration: 90 min
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Exposure 1

Main characteristics
Frequency 200 kHz
Type
Waveform
  • rectangular
Exposure duration 90 min
Modulation
Modulation type pulsed
Pulse width 1 µs
Packets per second 200,000
Exposure setup
Exposure source
Setup The sample was placed between the electrode plate.
Parameters

No parameters are specified for this exposure.

Additional parameter details

0.5 kV/m, 1.0 kV/cm, 1.5 kV/cm, 2.0 kV/m, 2.5 kV/m, 3 kV/cm and 3.5 kV/m peak-peak mean values.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The electron spin resonance spectrum of exposed rat liver homogenate appears mainly like the spectrum of the unexposed control. Except one outstanding signal which could be assigned as a indefinite mitochondrial free radical. The measurement of oxygen consumption in irradiated and sham-exposed probes revealed a correspondent respiratory activity of the mitochondria.

Study character:

Study funded by