EMF-Portal | Aneuploidy studies in human cells exposed in vitro to GSM-900 MHz radiofrequency radiation using FISH

Aneuploidy studies in human cells exposed in vitro to GSM-900 MHz radiofrequency radiation using FISH med./bio.

By: Bourthoumieu S, Terro F, Leveque P, Collin A, Joubert V, Yardin C

Published in: Int J Radiat Biol 2011; 87 (4): 400-408

Aim of study (acc. to author)

Endpoint

Exposure

Exposure	Parameters
Exposure 1: 900 MHz Modulation type: pulsed Exposure duration: continuous for 24 h	SAR: 4 W/kg spatial average (0.25, 1, 2, 4 W/kg) SAR: 32 W/kg peak value (2, 8, 16, 32 W/kg)

Exposure 1

Main characteristics

Frequency	900 MHz
Type	electromagnetic field
Exposure duration	continuous for 24 h

Modulation

Modulation type	pulsed
Pulse width	0.577 ms
Repetition frequency	217 Hz

Exposure setup

Exposure source	signal generator RFS900-60 + modulating unit RFPA
Setup	samples placed in eight 35 mm Petri dishes, each inside a 50 mm Petri dish; dishes positioned in a 15 cm x 15 cm wire-patch cell inside a box with absorbing walls of ferrite; exposure and sham exposure boxes kept in the same incubator
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
SAR	4 W/kg	spatial average	calculated	-	0.25, 1, 2, 4 W/kg
SAR	32 W/kg	peak value	calculated	-	2, 8, 16, 32 W/kg

Reference articles

Laval L et al. (2000): A new in vitro exposure device for the mobile frequency of 900 MHz

Methods Endpoint/measurement parameters/methodology

Investigated system:

chromosomes

Time of investigation:

after exposure

Main outcome of study (acc. to author)

No significant change in the rate of aneuploidy of chromosomes 11 and 17 was found following exposure to GSM-900 for 24 h at average SAR up to 4 W/kg. In conclusion, the data did not show any in vitro aneuploidogenic effect of GSM.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Fondation Santé et Radiofréquences, France

Pacchierotti F et al. (2011): Environmental hazard in the aetiology of somatic and germ cell aneuploidy

Bourthoumieu S et al. (2010): Cytogenetic Studies in Human Cells Exposed In Vitro to GSM-900 MHz Radiofrequency Radiation Using R-Banded Karyotyping

Verschaeve L et al. (2010): In vitro and in vivo genotoxicity of radiofrequency fields

Verschaeve L (2009): Genetic damage in subjects exposed to radiofrequency radiation

Vijayalaxmi et al. (2009): Genetic damage in mammalian somatic cells exposed to extremely low frequency electro-magnetic fields: a meta-analysis of data from 87 publications (1990-2007)

Mazor R et al. (2008): Increased levels of numerical chromosome aberrations after in vitro exposure of human peripheral blood lymphocytes to radiofrequency electromagnetic fields for 72 hours

Mashevich M et al. (2003): Exposure of human peripheral blood lymphocytes to electromagnetic fields associated with cellular phones leads to chromosomal instability