Medical/biological study (experimental study)

Effects of 2.45 GHz microwave exposures on the peroxidation status in Wistar rats med./bio.

By: Aweda MA, Gbenebitse S, Meidinyo RO

Published in: Niger Postgrad Med J 2003; 10 (4): 243-246

Aim of study (acc. to author)

To study the effect of microwave exposure on lipid peroxidation status in rats.

Background/further details

The effect of administration of the antioxidants ascorbic acid and alpha-tocopherol was also studied.
120 rats were divided into the following groups (each group = 5 rats): Six groups served as control group, six groups were microwave exposed, six groups were treated with ascorbic acid, and six groups were treated with alpha-tocopherol (both four days before exposure). The lipid peroxidation was monitored for a period of 8 weeks post irradiation.

Endpoint

plasma lipid peroxidation status in rats

Exposure

- 2.45 GHz
- microwaves

Exposure	Parameters
Exposure 1: 2.45 GHz Exposure duration: not given in the article	power density: 6 mW/cm² maximum

General information

For further information on the setup see also: Guy A. W, Dosimetry of Exposure to VLF to Microwaves, Health Physics. 1987; 56: 1 - 6.
Animals were divided into the following groups: i) microwave exposure ii) microwave exposure + 1 mg/kg ascorbic acid - 4 days before exposure iii) microwave exposure + 1 mg/kg alpha-tocopherol - 4 days before exposure iv) control

Exposure 1

Main characteristics

Frequency	2.45 GHz
Type	electromagnetic field
Exposure duration	not given in the article

Exposure setup

Exposure source	microwave generator Toshiba ER6660E + antenna
Distance between exposed object and exposure source	12 cm
Chamber	irradiation chamber surfaces lined with water

Parameters

Measurand	Value	Type	Method	Mass	Remarks
power density	6 mW/cm²	maximum	measured	-	-

Reference articles

Lai H et al. (1995): Acute low-intensity microwave exposure increases DNA single-strand breaks in rat brain cells
McRee DI et al. (1984): Whole-body and local dosimetry in rats exposed to 2.45-GHz microwave radiation

Exposed system:

animal
rat/Wistar
whole body

Methods Endpoint/measurement parameters/methodology

plasma lipid peroxidation status (malondialdehyde level, thiobarbituric acid method; spectrophotometry); rectal temperature (for SAR value calculation)

Investigated system:

blood samples
investigation on living organism

Time of investigation:

during exposure
after exposure

Main outcome of study (acc. to author)

The microwave exposure caused an increase in the lipid peroxidation within the first 24 hours after exposure, and then gradually reduced to the value of the control group after about one week. Ascorbic acid and alpha-tocopherol administration caused a decrease of the control value of the lipid peroxidation within the first week after irradiation.
The data demonstrated that microwave exposure caused significant increase in the lipid peroxidation status and there were protective effects of the antioxidants ascorbic acid and alpha-tocopherol.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

not stated/no funding

Chauhan P et al. (2017): Microwave radiation (2.45 GHz)-induced oxidative stress: Whole-body exposure effect on histopathology of Wistar rats
Ozgur E et al. (2013): Effects of Prenatal and Postnatal Exposure to GSM-Like Radiofrequency on Blood Chemistry and Oxidative Stress in Infant Rabbits, an Experimental Study
Naziroglu M et al. (2012): Melatonin modulates wireless (2.45 GHz)-induced oxidative injury through TRPM2 and voltage gated Ca(2+) channels in brain and dorsal root ganglion in rat
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