Medical/biological study (experimental study)

The effect of 50 Hz magnetic field of different shape on oxygen metabolism in blood platelets: in vitro studies med./bio.

By: Henrykowska G, Jankowski W, Pacholski K, Lewicka M, Smigielski J, Dziedziczak-Buczynska M, Buczynski A

Published in: Int J Occup Med Environ Health 2009; 22 (3): 269-276

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 15 min	magnetic flux density: 10 mT effective value

Frequency	50 Hz
Type	magnetic field
Exposure duration	continuous for 15 min
Additional info	fields with the following signal forms were applied: i) sinusoidal ii) triangular iii) rectangular

Exposure source	Helmholtz coils
Setup	pair of Helmholtz coils with a radius of 60 mm, 40 mm apart, producing an uniform field inside the 30 mm high cylindrical exposure volume with a radius of 32 mm; samples placed in polyethylene tubes in this exposure volume

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	10 mT	effective value	measured	-	-

Exposed system:

Investigated system:

Time of investigation:

The formation of free radicals, the concentration of malondialdehyde and the enzyme activity of catalase showed increased values compared with the initial levels, regardless of the shape of the magnetic field applied. The highest increase of free radical formation in relation to the initial value was found after exposure to the triangular magnetic field. Catalase enzyme activity also depended on the shape of the magnetic field: The highest increase was found after application of the triangular field, whereas the lowest one was observed when the sinusoidal magnetic field was applied. The highest increase of malondialdehyde concentration was found after sinusoidal magnetic field exposure compared to control.
Superoxide dismutase enzyme activity decreased after exposure and the enzyme activity depended on the shape of the magnetic field: The highest decrease, compared to the initial values, was observed when platelets were exposed to a magnetic field of triangular and rectangular shape.
The date indicate that the oxidative stress resulting from exposure to 50 Hz magnetic field may produce a number of adverse effects within the cell. Additionally, the findings point at the significance of the shape of the magnetic field.

Study character:

Lewicka M et al. (2015): The impact of electromagnetic radiation of different parameters on platelet oxygen metabolism - in vitro studies
Morabito C et al. (2010): Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach
Di Loreto S et al. (2009): Fifty hertz extremely low-frequency magnetic field exposure elicits redox and trophic response in rat-cortical neurons
Sharifian A et al. (2009): Effect of extremely low frequency magnetic field on antioxidant activity in plasma and red blood cells in spot welders
Jankowski W et al. (2008): [Influence of electromagnetic field on chosen parameters of thrombocytes' oxygen metabolism--in vitro research]
Calota V et al. (2007): Effects of prooxidants on human serum exposed to 50 Hz magnetic fields
Falone S et al. (2007): Fifty hertz extremely low-frequency electromagnetic field causes changes in redox and differentiative status in neuroblastoma cells
Wieraszko A et al. (2005): Neurophysiological Effects Induced in the Nervous Tissue by Low-Frequency, Pulsed Magnetic Fields
Zmyslony M et al. (2004): The effect of weak 50 Hz magnetic fields on the number of free oxygen radicals in rat lymphocytes in vitro