Medical/biological study (experimental study)

Effects of prooxidants on human serum exposed to 50 Hz magnetic fields med./bio.

By: Calota V, Dragoiu S, Meghea A, Giurginca M

Published in: Electromagn Biol Med 2007; 26 (2): 135-140

Aim of study (acc. to author)

This in vitro study should clarify whether the exposure to extremely low frequency magnetic fields induces alterations in free radical formation in human serum.

Background/further details

The sera of a 34 year old woman and a 40 year old man, both non smoker and healthy, were used. Additionally, the oxidants iron (II)-chloride (FeCl₂) and H₂O₂ were applied in three different concentrations (1 µM, 5 µM, 10 µM).

Endpoint

molecular biosynthesis: free radical formation

Exposure

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuous for 1 h	magnetic flux density: 0.357 mT magnetic flux density: 0.596 mT magnetic flux density: 1.788 mT magnetic flux density: 2.384 mT
Exposure 2: 50 Hz Exposure duration: continuous for 1 h or 2 h	magnetic flux density: 0.357 mT (for the female serum) magnetic flux density: 2.384 mT (for the male serum)

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Exposure duration	continuous for 1 h

Exposure setup

Exposure source	Helmholtz coils
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.357 mT	-	-	-	-
magnetic flux density	0.596 mT	-	-	-	-
magnetic flux density	1.788 mT	-	-	-	-
magnetic flux density	2.384 mT	-	-	-	-

Exposure 2

Main characteristics

Frequency	50 Hz
Type	magnetic field
Exposure duration	continuous for 1 h or 2 h
Additional info	prooxidants FeCl₂ and H₂O₂ in different concentrations added

Exposure setup

Exposure source	same setup as exposure 1
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.357 mT	-	-	-	for the female serum
magnetic flux density	2.384 mT	-	-	-	for the male serum

Exposed system:

serum

Methods Endpoint/measurement parameters/methodology

molecular biosynthesis: free radical formation (intensitiy of chemiluminescence)

Investigated system:

serum

Time of investigation:

before exposure
during exposure
after exposure

Main outcome of study (acc. to author)

Female and male serum behaved in different manner due to exposure. Whereas male serum showed increasing chemiluminescence with increasing magnetic flux densities, the maximal chemiluminescence in female serum was observed at minimum magnetic flux density.
The treatment with the oxidants increased the chemiluminescence even more. Whereas magnetic field exposed serum of the man exhibited increased chemiluminescence with increasing oxidant concentrations (FeCl₂ more pronounced than H₂O₂), one hour exposed serum of the woman behaved vice versa.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

not stated/no funding

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Effects of prooxidants on human serum exposed to 50 Hz magnetic fields med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

Exposure

Exposure 1

Main characteristics

Exposure setup

Parameters

Exposure 2

Main characteristics

Exposure setup

Parameters

Methods Endpoint/measurement parameters/methodology

Main outcome of study (acc. to author)

Study funded by

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