Study type: Medical/biological study (experimental study)

Entamoeba invadens: influence of 60 Hz magnetic fields on growth and differentiation med./bio.

By: Rodriguez de la Fuente AO, Heredia-Rojas JA, Mata-Cardenas BD, Vargas-Villarreal J, Rodriguez-Flores LE, Balderas-Candanosa I, Alcocer-Gonzalez JM
Published in: Exp Parasitol 2008; 119 (2): 202-206
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Aim of study (acc. to author)

To study the effects of 60 Hz sinusoidal magnetic fields at different magnetic flux densities on growth and differentiation of the protozoan Entamoeba invadens.

Background/further details

Throphozoite cultures were exposed for 9 or 10 days to 60 Hz magentic fields. In order to test the combination of metronidazole (a cytotoxic drug against amoebic cells) and 60 Hz magnetic field on throphozoite growth, a co-exposure experiment was conducted with four groups: 1) sham-exposed group, 2) cells treated with metronidazole LD50 (positive control), 3) cells exposed for seven days to 2 mT magnetic fields, and 4) cells treated with 2 mT magnetic field plus metronidazole LD50.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 9 days with glucose during encystation process, continuous for 10 days without glucose during encystation process
Exposure 2: 60 Hz
Exposure duration: continuous for 7 days

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 9 days with glucose during encystation process, continuous for 10 days without glucose during encystation process
Exposure setup
Exposure source
Setup 71 cm long solenoid with a radius of 13.5 cm, made of 552 turns of 1.3 mm enamel-insulated copper wire; cultures placed in the middle of the solenoid
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT effective value measured - -
magnetic flux density 1.5 mT effective value measured - -
magnetic flux density 2 mT effective value measured - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration continuous for 7 days
Additional info co-exposure
Additional info cultures were treated in four groups: i) sham exposure ii) cells treated with Metronidazole LD50 iii) MF exposure iv) cells treated with Metronidazole LD50 + MF exposure
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT effective value measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

An inhibitory effect on growth (decreased cell densities) was found when throphozoite cultures were exposed to 1.5 and 2 mT. There was no synergistic effect in cultures co-exposed to magnetic fields and metronidazole. Magnetic field exposure (alone) inhibited the encystation process of Entamoeba invadens.
Magentic field exposure of the protozoan Entamoeba invadens altered the growth and encystation process.

Study character:

Study funded by

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