Medical/biological study (experimental study)

ELF non ionizing radiation changes the distribution of the inner chemical functional groups in human epithelial cell (HaCaT) culture med./bio.

By: Lisi A, Rieti S, Cricenti A, Flori A, Generosi R, Luce M, Perfetti P, Foletti A, Ledda M, Rosola E, Giuliani L, Grimaldi S

Published in: Electromagn Biol Med 2006; 25 (4): 281-289

Download citation in RIS format

Aim of study (acc. to author)

This in vitro study was performed to investigate changes in morphology and phospholipid/protein distribution of sinusoidal extremely low frequency electromagnetic field exposed human keratinocytes.

Background/further details

The temperature was continuously measured and maintained at the range of 37 +/- 0.3°C.

Endpoint

morphol./histopathol. changes: cell morphology, protein/phospholipid distribution

Exposure

- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: 24 h	magnetic flux density: 1 mT effective value electric field strength: 0.01 µV/cm

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	24 h

Exposure setup

Exposure source	solenoid
Setup	solenoid with an inner diameter of 20 cm and a height of 40 cm consisting of 2 cm thick concrete-asbestos with 1.200 turns of 2 mm copper wire; exposure volume along the solenoid axis: 11 x 17 cm; temperature control
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1 mT	effective value	measured	-	-
electric field strength	0.01 µV/cm	-	calculated	-	-

Reference articles

Santoro N et al. (1997): Effect of extremely low frequency (ELF) magnetic field exposure on morphological and biophysical properties of human lymphoid cell line (Raji)
Stauffer PR et al. (1994): Practical induction heating coil designs for clinical hyperthermia with ferromagnetic implants
Ellinger DC et al. (1989): Evaluation of a semi-cylindrical solenoid as an applicator for radio-frequency hyperthermia

Exposed system:

intact cell/cell culture
HaCaT cells (human keratinocytes cell line)

Methods Endpoint/measurement parameters/methodology

morphol./histopathol. changes: cell morphology, protein/phospholipid distribution (infrared-scanning near-field optical microscopy (IR-SNOM))

Investigated system:

isolated bio./chem. substance
intact cell/cell culture

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Electromagnetic field exposed cells exhibited more flattened morphology compared to control group.
Exposed cells showed an increase of newly synthesized protein, jointly with a different phospholipid distribution.

Study character:

medical/biological study
experimental study
full/main study
blind study

Study funded by

Istituto Superiore per la Prevenzione e la Sicurezza del Lavoro (ISPESL; National Institute for Occupational Safety and Prevention), Italy

Patruno A et al. (2015): mTOR Activation by PI3K/Akt and ERK Signaling in Short ELF-EMF Exposed Human Keratinocytes
Ahmadian S et al. (2006): Effects of extremely-low-frequency pulsed electromagnetic fields on collagen synthesis in rat skin
Rajkovic V et al. (2005): Histological characteristics of cutaneous and thyroid mast cell populations in male rats exposed to power-frequency electromagnetic fields
Rajkovic V et al. (2005): The effect of extremely low-frequency electromagnetic fields on skin and thyroid amine- and peptide-containing cells in rats: an immunohistochemical and morphometrical study
Manni V et al. (2004): Low electromagnetic field (50 Hz) induces differentiation on primary human oral keratinocytes (HOK)
Rieti S et al. (2004): SNOM and AFM microscopy techniques to study the effect of non-ionizing radiation on the morphological and biochemical properties of human keratinocytes cell line (HaCaT)