Study type: Medical/biological study (experimental study)

The influence of magnetic fields exposure on neurite outgrowth in PC12 rat pheochromocytoma cells med./bio.

By: Fan W, Ding J, Duan W, Zhu YM
Published in: J Magn Magn Mater 2004; 282: 325-328
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Aim of study (acc. to author)

To study the influence of magnetic field exposure on neurite outgrowth in PC12 cells. These cells can react to the addition of the nerve growth factor by differentiating and growing neurites.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: continuous for 96 h
Exposure 2: 50 Hz
Modulation type: pulsed
Exposure duration: 96 h

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration continuous for 96 h
Modulation
Modulation type unspecified
Exposure setup
Exposure source
Chamber 5% CO2 incubator maintained at 37°C
Setup Dishes containing PC12 cells with 30 ng/ml NGF (nerve growth factor) were placed in the exposure system
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 230 µT - - - -

Exposure 2

Main characteristics
Frequency 50 Hz
Type
Exposure duration 96 h
Modulation
Modulation type pulsed
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1.32 mT - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

A promotion was found under a weak magnetic field (0.23 mT), as the average number of neurites per cell increased compared to neurites/cell of the controls, while inhibition and directional outgrowth was evident under relatively stronger magnetic field (1.32 mT). The study shows that biological systems can be very sensitive to the strength of electromagnetic field.

Study character:

Study funded by

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