Isolated somatic embryos of Quercus suber were chronically exposed to a 50 Hz and 15 µT electromagnetic field in order to study if the extremely low frequency magnetic field affected the mophogenic behaviour of embryonic cultures (proliferation of embryonic cultures, differentiation of somatic embryos, and germination of somatic embryos) during recurrent embryogenesis. Additionally, germination of somatic embryos was carried out under the same electromagnetic field, and also under conditions in which the local geomagnetic field was suppressed.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continuous for 8 weeks
|
|
|
Exposure 2:
Exposure duration:
continuous for 8 weeks
|
- |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 8 weeks |
| Exposure source | |
|---|---|
| Setup | Somatic embryos were placed into open glass wells which were placed in closed Magneta vessels. Plastic trays containing Magneta vessels were placed in the geomagnetic center of the coils. |
| Additional info | The applied field was perpendicular to geomagnetic field. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 15 µT | effective value | measured | - | - |
| Frequency | |
|---|---|
| Type | |
| Exposure duration | continuous for 8 weeks |
| Exposure source | |
|---|---|
| Setup | Helmholtz coils were arranged with their axis parallel to the total geomagnetic field (43 µT). A dc current was applied to the coils and regulated so that the generated field was opposed and had the same magnitude as the geomagnetic field. |
No parameters are specified for this exposure.
The extremely low frequency magnetic field did not influence the growth of embryonic clumps of the assayed genotypes, but reduced the number of detachable embryos. The extremely low frequency magnetic field did not change the percentages of germination or plant formation of somatic embryos. Somatic embryos had better germination when cultured under the suppressed geomagnetic field condition.
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