Study type: Medical/biological study (experimental study)

Microwave and thermal interactions with oxidative hemolysis med./bio.

By: Kiel JL, Erwin DN
Published in: Physiol Chem Phys Med NMR 1984; 16 (4): 317-323
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Aim of study (acc. to author)

To investigate the effects of 2450 MHz (2.45 GHz) microwave irradiation on spontaneous hemolysis of human erythrocytes in vitro. Some of these cells were treated with CDNB (1-chloro-2,4-dinitrobenzene) and/or coated with WGA-HRP conjugate (wheat germ agglutinin-horseradish peroxidase conjugate) for sensing oxidative damage.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: pulsed
Exposure duration: 20 min

Exposure 1

Main characteristics
Frequency 2.45 GHz
Exposure duration 20 min
Modulation
Modulation type pulsed
Duty cycle 0.02 %
Repetition frequency 3,333 Hz
Exposure setup
Exposure source
Setup Cell suspensions were held in a 1.2 cm x 5.1 cm cellulose nitrate tube which was contained in a polystyrene foam test tube holder placed in the waveguide.
Parameters
Measurand Value Type Method Mass Remarks
SAR 400 µW/g mean unspecified - -
power density 50 W/m² mean measured - -

Reference articles

  • Guy AW et al. (1979): Circularly polarized 2450 MHz waveguide system for chronic exposure of small animals to microwaves

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Microwave exposure significantly decreased spontaneous hemolysis of untreated human erythrocytes at 42°C, but had no effect at 37°C or 48°C. Microwave exposure significantly enhanced a CDNB membrane stabilizing effect at 42°C but not at 37°C or 48°C. WGA-HRP coated cells showed an increased hemolysis under microwave exposure at 42°C, whereas cells treated with both WGA-HRP and CDNB showed no fragility at 42°C and increased fragility at 48°C without a microwave effect.

Study character:

Study funded by

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