Study type: Medical/biological study (experimental study)

Influence of CW microwave radiation on in vitro release of enzymes from retinol- treated hepatic lysosomes med./bio.

By: Galvin MJ, Parks DP, MacNichols G, McRee DI
Published in: Cell Biophys 1981; 3 (2): 175-186
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Aim of study (acc. to author)

To examine the effects of microwave exposure on the release of lysosome-specific enzymes accompanied by retinol treatment in vitro.

Background/further details

The fat soluble compound retinol (vitamin A) effects directly the lysosomal membrane which leads to the release of the hydrolases contained in the lysosomes. A possible synergistic effect between microwave exposure and retinol treatment should be investigated. The lysosomes were isolated from rat liver tissue. The studied enzymes were β-glucuronidase, acid phosphatase, and cathepsin D.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: continuous for 60 min
with retinol added
Exposure 2: 2.45 GHz
Modulation type: CW
Exposure duration: 40 min prior to addition of retinol, 20 min after addition of Retinol
Exposure 3: 2.45 GHz
Modulation type: CW
Exposure duration: continuous for 80 min
with retinol

Exposure 1

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration continuous for 60 min
Additional info with retinol added
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup glass sample tube placed inside the waveguide against the dielectric plate
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 10 mW/g - calculated - -
SAR 100 mW/g - calculated - -

Exposure 2

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration 40 min prior to addition of retinol, 20 min after addition of Retinol
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 10 mW/g - calculated - -
SAR 100 mW/g - calculated - -

Exposure 3

Main characteristics
Frequency 2.45 GHz
Type
Exposure duration continuous for 80 min
Additional info with retinol
Modulation
Modulation type CW
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 10 mW/g - calculated - -
SAR 100 mW/g - calculated - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The microwave exposure had no influence on the retinol-induced enzyme release in lysosomes compared to unexposed control probes.

Study character:

Study funded by