Study type: Medical/biological study (experimental study)

Microwave radiation effects on cardiac muscle cells in vitro med./bio.

By: Galvin MJ, Hall CA, McRee DI
Published in: Radiat Res 1981; 86 (2): 358-367
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Aim of study (acc. to author)

To investigate the effects of 2450 MHz CW microwave exposure on embryonic heart muscle cells of japanese quail in vitro.

Background/further details

A maximum temperature increase of 0,5°C was observed at SARs of 100 mW/g. Intact cells will exclude trypan blue, thus an uptake of this dye reveals altered membrane properties. The release of intracellular enzymes, such as LDH or creatine phosphokinase, serve as an indicator of abnormal membrane permeability as well.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 2.45 GHz
Modulation type: CW
Exposure duration: 90 min
  • SAR: 1 mW/g average over time
  • SAR: 10 mW/g average over time
  • SAR: 50 mW/g average over time
  • SAR: 100 mW/g average over time

Exposure 1

Main characteristics
Frequency 2.45 GHz
Exposure duration 90 min
Modulation
Modulation type CW
Exposure setup
Exposure source
Setup The sample tube (1.05/1.36 cm inner/outer diameter) with magnetic stirrers was placed against the dielectric plate in the waveguide filled with distilled water and maintained at 37°C.
Additional info the control sample tube was placed 9.5 cm away from the dielectric plate and the temperature was the same as in the case of the exposed samples
Parameters
Measurand Value Type Method Mass Remarks
SAR 1 mW/g average over time calculated - -
SAR 10 mW/g average over time calculated - -
SAR 50 mW/g average over time calculated - -
SAR 100 mW/g average over time calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

It was found that even low SARs of 10 mW/g increased the uptake of trypan blue and at SARs of 100 mW/g a release of LDH was observed. Electron microscopy confirmed cellular damage only at SAR values of 100 mW/g.

Study character:

Study funded by