EMF-Portal | Behaviour of monolayer cultures exposed to a low-frequency modulated radiofrequency electric field

Behaviour of monolayer cultures exposed to a low-frequency modulated radiofrequency electric field med./bio.

By: Mueller M, Maile S, Merker HJ, Kramer KD

Published in: Bioelectrochem Bioenerg 1995; 37 (1): 11-16

Aim of study (acc. to author)

To investigate the effect of a radiofrequency electric field on dye take-up and storage by murine fibroblasts cells.

Endpoint

cell function: dye take-up and storage

Exposure

Exposure	Parameters
Exposure 1: 150 MHz Modulation type: AM Exposure duration: 10 runs of 3 h each	electric field strength: 90 V/m effective value (constant)

Exposure source	cavity resonator
Chamber	TE101 cavity resonator, sampel holder, eiter 3 multi well plates ( 60mm high)with 96 vessels/plate or 3 flasks 50 x40mm HxD
Additional info	Half of the samples were exposed while the other half remained in the incubator for positive.

Measurand	Value	Type	Method	Mass	Remarks
electric field strength	90 V/m	effective value	measured	-	constant

Exposed system:

Methods Endpoint/measurement parameters/methodology

cell function: cell ability of take-up and lysosomal storage of the dye neutral red (photometric measurement at 540 nm)

Investigated system:

Time of investigation:

Main outcome of study (acc. to author)

The dye take-up by the cells reveals a maximum near 16 Hz amplitude modulation. However, this effect is not very marked.

Study character:

Study funded by

not stated/no funding