Medical/biological study (experimental study)

A novel method for the study of fluorescent probes in biological material during exposure to microwave radiation med./bio.

By: Ortner MJ, Galvin MJ, McRee DI, Chignell CF

Published in: J Biochem Biophys Methods 1981; 5 (3): 157-167

Journal partially peer-reviewed/peer-review process unclear

Exposure	Parameters
Exposure 1: 2.45 GHz Modulation type: CW Exposure duration: continuous 10 min	SAR: 10 mW/g minimum (center of stirred solution) SAR: 200 mW/g maximum (center of stirred solution)

Exposure 1

Main characteristics

Frequency	2.45 GHz
Type	electromagnetic field
Waveform	sinusoidal
Polarization	linear
Exposure duration	continuous 10 min

Modulation

Modulation type	CW

Exposure setup

Exposure source	circular waveguide
Chamber	waterfilled

Parameters

Measurand	Value	Type	Method	Mass	Remarks
SAR	10 mW/g	minimum	measured	-	center of stirred solution
SAR	200 mW/g	maximum	measured	-	center of stirred solution

Exposed system:

human erythrocyte ghost

Methods Endpoint/measurement parameters/methodology

ANS and calcium binding; intermolecular distance between ANS and tryptophan residues (spectrophotometry)

Investigated system:

as exposed system

Time of investigation:

during exposure

Main outcome of study (acc. to author)

Overall no effects were observed. Neither specific absorption rates of 10 and 200 mW/g nor calcium doses between 2.0 and 10.0 x10^-4 M had any effect on binding of ANS to the erythrocyte ghosts or calcium binding, respectively. Also the intrermolecular distances between membrane bound ANS and intrinsic tryptophan residues were not altered.

Study character: