This study was performed to evaluate the effects of nanosecond pulsed electric fields on membrane permeabilization of mammalian cells in vitro.
In a first step an experimental setup for nanosecond pulsed electric fields exposure of cell cultures using the patch-clamp technique was developed. Thereafter the cell lines were exposed to a single 60 nanosecond pulse or to multiple 60 nanoseconds pulsed electric fields in different ion milieus. 6-12 experiments were conducted per group.
Exposure | Parameters |
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Exposure 1:
Modulation type:
single pulse
Exposure duration:
single pulse of 60 ns
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Modulation type | single pulse |
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Pulse width | 60 ns |
Exposure source |
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Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
electric field strength | 12 kV/cm | - | calculated | - | at cell location |
electric field strength | 22 kV/cm | minimum | calculated | - | between electrodes |
electric field strength | 24 kV/cm | maximum | calculated | - | between electrodes |
Even a single 60 nanoseconds pulse at 12 kV/cm could cause a profound and long-lasting (at least 100 seconds) reduction of the cell membrane resistance, accompanied by the loss of the membrane potential. Membrane resistance decreased in all investigated cell lines except for the human cervical carcinoma cells. Its recovery could take 15 minutes. Multiple electric pulses also enhanced the cell membrane permeabilization.
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