Study type: Medical/biological study (experimental study)

Lethal and inhibitory effects of steep pulsed electric field on tumor-bearing BALB/c mice med./bio.

By: Mi Y, Sun C, Yao C, Xiong L, Liao R, Hu Y, Hu L
Published in: The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, San Francisco, CA, USA. IEEE, 2004: pp. 5005-5008; ISBN 978-0-7803-8439-2
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Aim of study (acc. to author)

This in vivo study examined whether the exposure to pulsed electric fields exerts lethal and inhibitory effects on tumor-bearing mice.

Background/further details

48 female tumor-bearing mice (26 animals in the exposure group and 22 animals in the control group) in the age of 10 to 12 weeks weighing 25 to 28 gramms were used. Exposure was performed when the tumor diameter had reached at least 9 mm.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Modulation type: pulsed
Exposure duration: 30 min

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Exposure duration 30 min
Modulation
Modulation type pulsed
Rise time 160 ns
Additional info

pulse width: 25 - 37 µs
Exposure setup
Exposure source
Setup electrodes were placed subcutaneously on opposite sides of the tumor
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
cf. remarks - - - - voltage: 40 V

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The histological tumor examination showed that pulsed electric field exposure destroyed the integrity of tumor cells irreversibly visible in nucleus changes: chromatin dissolution, pyknosis, nucleus fragmentation. The tumor growth in exposed animals was significantly slowed down. The survival period was higher in the exposure group (52 days) than in the control group (33 days).

Study character:

Study funded by

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