Medical/biological study (experimental study)

Suppression of natural killer cell activity on Candida stellatoidea by a 50 Hz magnetic field med./bio.

By: Canseven AG, Seyhan N, Mirshahidi S, Imir T

Published in: Electromagn Biol Med 2006; 25 (2): 79-85

Aim of study (acc. to author)

To study natural killer cell activity in splenocytes (splenic cells) of guinea pigs (10 exposed; 9 controls) after exposure to a 50 Hz magnetic field.

Endpoint

effects on the immune system: natural killer cell activity

Exposure

- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: repeated daily exposure, 4 h/day for 5 days	magnetic flux density: 2 mT

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	repeated daily exposure, 4 h/day for 5 days

Exposure setup

Exposure source	Helmholtz coils circular coil pairs: 42.75 cm diameter, 24.375 cm clearance made of 154 turns of copper wire
Setup	10 guinea pigs were housed in the center of the coils, 2 per plastic cage; magnetic field lines perpendicular to the bottom plane of the animal`s cage
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	2 mT	-	measured	-	-

Exposed system:

animal
guinea pig
whole body

Methods Endpoint/measurement parameters/methodology

cell function: natural killer cell cytotoxic activity (see "effects on immunological system")
effects on the immune system: natural killer cell activity (Colony Forming Inhibition Assay with the yeast Candida stellatoidea as target cells; number of colonies)

Investigated system:

intact cell/cell culture

Investigated organ system:

immune system

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Natural killer cell cytotoxic activity was decreased in exposed animals compared to the control group. The data suggests that natural killer cell activity can be suppressed by a 50 Hz magnetic field.

Study character:

medical/biological study
experimental study
full/main study
blind study

Study funded by

not stated/no funding

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