Study type: Medical/biological study (experimental study)

The relationship between electropermeabilization and cell cycle and cell size of Saccharomyces cerevisiae med./bio.

By: Hojo S, Shimizu K, Yositake H, Muraji M, Tsujimoto H, Tatebe W
Published in: IEEE Trans Nanobioscience 2003; 2 (1): 35-39
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Aim of study (acc. to author)

To investigate the cell membrane dye-permeability caused by an application of electric pulses in relation to cell cycle and cell size of the yeast Saccharomyces cerevisiae

Background/further details

Yeast cells in a dye containing solution were treated with electric pulses. The membrane permeability to the dye was determined in relation to cell cycle and cell size.

Endpoint

Exposure

Exposure Parameters
Exposure 1:
Modulation type: pulsed
Exposure 2:
Modulation type: pulsed

Exposure 1

Main characteristics
Type
Waveform
  • rectangular
Additional info Electric pulses were applied to yeast cell suspensions in their logarithmic growth phase
Modulation
Modulation type pulsed
Pulse width 50 µs
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 371 kV/m minimum unspecified - 429 kV/m, 486 kV/m and 543 kV/m

Exposure 2

Main characteristics
Type
Waveform
  • rectangular
Additional info Electric pulses were applied to yeast cell suspensions in their stationary phase
Modulation
Modulation type pulsed
Pulse width 50 µs
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 486 kV/m minimum unspecified - 529 kV/m, 571 kV/m and 614 kV/m

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

It was found that cells from S-phase to mitosis-phase in the cell cycle tend to be more dye-permeable than cells in G1-phase. Irrespective of the cell cycle the dye-permeability decreases with increasing cell size.

Study character:

Study funded by